Learning Outcomes

Understanding the Basics of HPLC

Historical Development of HPLC

Advantages and Applications of HPLC

HPLC Components and Principles of Operation

Principles of Separation, Mobile and Stationary Phase Interactions

Types of HPLC

Elution Types

Lesson Summary

In this module, you will be introduced to the sophisticated analytical technique of HPLC. First, we will define HPLC in ways you will understand. Secondly, you will learn the historical development and importance of HPLC. Then, we will walk you through the applications of HPLC in pharmaceuticals, food, and environmental analysis.

Setting Up the HPLC System

HPLC Sample and Mobile Phase Preparation

Running an HPLC Analysis

In this module, you will learn how to operate High-Performance Liquid Chromatography. We will start by walking you through the setup of an HPLC system so that you can be familiar with the components. Then, you learn how to prepare your sample for analysis, after which you will learn how to start and complete a full run analysis on the HPLC system.

Introduction to HPLC Troubleshooting

Common Problems in HPLC

Developing a Troubleshooting Strategy

In this module, you will learn how to troubleshoot HPLC systems. First, you will be introduced to common HPLC problems like baseline noise, pressure fluctuations, ghost peaks, and sample carryover. Then, we will teach you how to apply systematic troubleshooting techniques to resolve issues related to the mobile phase, column clogging, and detector.

Routine and Preventive Maintenance

Cleaning of HPLC System

Best Practices for Long-term HPLC Performance

In this module, you will learn how to maintain HPLC systems. First, you will learn routine preventive maintenance of the HPLC system which involves 1. Column care: Regeneration, storage, and replacement. 2. Cleaning the injector, pump, and detector components. Then, you will learn best practices to adopt to protect the shelf-life of HPLC system.

Mastering HPLC - Operation, Troubleshooting and Maintenance - Course Assessment

Command respect and become an expert in HPLC operation, troubleshooting and maintenance in this free online course.

Mastering HPLC: Operation, Troubleshooting, and Maintenance

This course provides a comprehensive introduction to High-Performance Liquid Chromatography (HPLC), covering its operation, key components, and practical usage in laboratory settings. It will equip you with the skills to troubleshoot common issues and maintain the equipment for optimal performance, making it the best choice for professionals and students in analytical chemistry, pharmaceuticals, foods, and related fields.

Paper Chromatography

Thin-Layer & High-Performance Liquid Chromatography

Gas-Liquid Chromatography

This module investigates different chromatography techniques, each with its specific applications. Along with other methods, you'll learn about paper chromatography, two-way chromatography, thin-layer chromatography, and their underlying separation principles.

Nuclear Magnetic Resonance (NMR)

Types of 1H-NMR

Carbon-13 NMR Spectroscopy

In this module, we explore a powerful spectrometry analytical technique known as nuclear magnetic resonance (NMR) and the properties that NMR studies. Carbon-13 NMR spectroscopy, proton (1H) nuclear magnetic resonance and the two types of 1H-NMR: low- and high-resolution NMR are also explained.

Mass Spectrometry

High-Resolution Mass Spectrometry

A Mass Spectrometer as a Combined Technique

This module discusses the mass spectrometer, a powerful tool for determining isotope abundance. We describe the steps of mass spectrometry sample analysis and low- and high-resolution mass spectrometry techniques. You'll discover how mass spectrometers can be used in tandem with other methods to identify organic compounds.

Introduction to Designing Medicinal Drugs

Chirality in Pharmaceutical Synthesis

Increasing the Number of Carbons in a Compound

This module covers medicinal drug design and chirality in pharmaceutical synthesis. We investigate molecular modelling, utilised successfully in the development of cancer treatments and HIV therapy. You'll also learn about chiral carbon, enantiomers, pure enantiomer preparation methods, and retrosynthesis stages.

Advanced Chemistry: Analytical Chemistry and Organic Synthesis - Course Assessment

This free online science course explains how to detect and identify chemical substances and discusses drug design.

Advanced Chemistry: Analytical Chemistry and Organic Synthesis

There are many techniques used to detect and identify chemical substances. This advanced chemistry course describes specialised instruments used in analytical chemistry, such as gas-liquid chromatography, nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. We also look into various methods used to design pharmaceuticals, including molecular modelling and X-ray crystallography, and explain how to prepare pure enantiomers.

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